Based on comprehensive analysis of field work, petrological and geochemical characteristics, this paper studies the characteristics and origin of giant patch dolomite of the Lower-Middle Ordovician HangGuletag Formation in Wuligezitag area, NE Tarim Basin, China. It can be seen in the field that the grayish yellow giant patch dolomite partially develops in the black thin-bedded micritic limestone, with an irregular boundary not controlled by sedimentation. The giant patches are several meters in size (the largest >10 m) which is larger than and quite different from the several-centimeter (decimeter) burrow-related dolomite or leopard fur dolomite. Microscopically, it consists of micro-finely and planare(s) dolomite crystals with a size near 100 μm. Besides, it is also characterized by its unique geochemical features: 1) δ13CPDB (−1.34‰-−0.62‰) accords with the range of Early Ordovician seawater while δ18OPDB (−8.01‰ -−4.79‰) is higher; 2) 87Sr/86Sr (0.711000-0.711535, AVG=0.710863) is much higher than that of limestone and contemporaneous seawater; 3) average high Fe and Mn content (2001.32 and 601.73 μg/g, respectively) and low Sr and Ba (33.14 and 8.27 μg/g, respectively) content versus that of limestone; 4) dolomite has slightly negative Eu anomaly and similar REE composition and distribution patterns compared with limestone; 5) low order degree value (AVG=0.6). This study reveals that the dolomitization was related to fault and occurred in low-temperature and shallow-burial environment in Early-Middle Ordovician. Dolomitizing fluid was generated from the concentrated formation fluid in lower-middle member of HangGuletag Formation and underlying Tursaktag Group. Most Mg2+ came from the stabilization of quasi-stable carbonate minerals, and the transformation of clay minerals of (calcareous) mud in the limestone strata not only provided a little Mg2+ but caused the rise of 87Sr/86Sr. Dolomitizing fluid was likely to be richened in Xingdi-related normal fault and fractures which also acted as the main fluid pathways, and it finally caused the partial dolomitization of nearby limestone strata.